0 out of 5 $ 11,999. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Click SoftMax Pro Software User Guide. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. The plate reader can measure multiple readouts, including UV and visible absorbance. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Additionally, user-installable options allow the SpectraMax i3 System to. Reader modes are customized to your specific applications with options to upgrade easily. The powerful analysis tools in the software generate a standard curve and calculated data. A new technical approach to platelet aggregation studies. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Molecular Interactions. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Introduction. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Molecular Devices: Molecular Devices. 313 reference gain check fail. Three low volume 24-well bottom slides. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Download the SpectraMax Mini Reader Plug-in and associated guides. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. B: Selection of Excitation Wavelength. Omnia® Compatible Microplate Reader Documentation Version No. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. These lab workhorses are used in drug discovery, research, bioassay. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. The chamber contains springs that position the cuvette in the proper alignment for a read. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. 100 fmol 1536-well (8 µL) 0. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. . 012 fmol 0. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. SpectraMax Microplate Readers. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. The system uses two scanning monochromators to determine the optimal excitation and emission settings. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. . Endpoint, kinetic, spectrum. flexibility of monochromator-based systems. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Example: [Drawer]. Microplate reader settings. Self Service Available. 304 signal level saturation. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Published Dec 16, 2020 | Updated Dec 16, 2020. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. Microplate Readers Biotek Epoch Microplate Reader. Detection modalities include absorbance (UV-Vis Abs) and. Our specialization servicing. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. . It features a modular design that allows users to add or change detection cartridges according to their needs. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraDrop microplate was read on the SpectraMax ® Paradigm ® Multi-Mode Microplate Reader using the Fluorescence Intensity detection cartridge (FI-FLRH) with fluorescein filters, or Tunable Wavelength Selection (TUNE) cartridge with signal/ background-optimized wavelengths. e. 72 MB. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. The drawing shows a standard microplate of 15 mm height covered with a lid. We use Spectramax M5 microplate reader in our lab. A: Microplate Preparation. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. 314 low lamp level warning. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. microplate reader. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. The dual monochromator optics allow the. Path Check Pathlength Measurement every Gemini EM Reader. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 2. 313 reference gain check fail. Unlock your i3/i3x instrument after shipment. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. . 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). It measures the optical density (OD) of samples in both 96- and 384-well. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 20 μL of Component A was added to each well of the microplate. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Microplate readers’ standard detection modes. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Products : General, Microplate Readers. Example: [Drawer]. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. The system uses two scanning monochromators to determine the optimal excitation and emission settings. We would like to show you a description here but the site won’t allow us. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Intended Use/Discipline:Biological. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. Detailed specifications of each model reader are reported in Section. On the back, in the right hand top corner,. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. 1. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Type:Spectrophotometer. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The dual monochromator optics allow. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. To resume collecting kinetic measurements within the same file, press the Append button. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. 470 nm). Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. microplate reader. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Category 2 and 3 microplate readers use gradient heating as well. Materials. Molecular Devices. . Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The screw remains inside the instrument. A. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. When the user applies a set temperature of 37°C, the top is heated to 37. 1. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). zip. Italic and boldface type are used for emphasis. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. With optimized reagents, option to operate the. Step 4: Incubate at room temperature for 1 hour, protected from light. Step 2: Launch SoftMax Pro Software. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 0 out of 5 $ 9,995 $ 9,495. 40266 Email: drugdiscoverytech@lifetech. The calculated LOQ values were 0. . Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The Gemini EM can sample a variety of formats, from 6- to 384-well. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. SoftMax® Pro 7. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Fluorescence Microplate Reader Comparison Chart. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. For a higher . The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Users can perform spectral scans from 250 nm to 750. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. ATP standard curves read on SpectraMax microplate readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. When the user applies a set temperature of 37°C, the top is heated to 37. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Transcreener ® ADP. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Data exemplifying linear ranges from the three different kits are presented in Figure 2. If it looks fine, then proceed. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Introduction. The plate. 7°C and the bottom to 36. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 315 can't find zero order. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). 0 out of 5 $ 11,999. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. ATP standard curves read on SpectraMax microplate readers. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Contents of wells were pipetted up and down several times to mix. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Examples: “Press carefully to engage,” “ Do not. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. We would like to show you a description here but the site won’t allow us. SpectraMax iD5 Multi-Mode Microplate Reader. keeping with best practices, reader performance should. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). In addition, the SpectraMax iD5 can be expanded to. Brochure. The guide also covers the software features, the specifications, and the accessories of the systems. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. Highly customizable low volume. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Figure 3. SpectraMax Microplate Readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Step 2: Launch SoftMax Pro Software. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Offer Ends In: 18 Days 19: 38: 21. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. 314 low lamp level warning. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. . High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. Wide range of applications The VersaMax reader covers. Molecular Devices Spectramax iD3 Microplate Reader. View the Molecular Devices website. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). Also see:. Step 1: Open the SoftMax Pro IMAP FP protocol. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. . The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. Most papers they cultured bacteria to OD600>0. 1 software Download page. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 303 unable to cal dark current. Cell Cytotoxicity assay procedure. Each sample has a discrete sample beam and reference beam so that each well is measured directly. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The SpectraMax M2 and M2e are versatile and reliable. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. Temperature control. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Sensitive fluorescent quantitation of DNA with the Quant-iT. Lysozyme contains two tryptophan residues that. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. 76358-624. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). Multimode Microplate Readers. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. their consistent performance, reliability and durability. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. . Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Figure 3. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. SAN JOSE, CA — Molecular Devices, LLC. See SpectraMax® Microplate Reader User Manual Download Page. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. Order Now. MPN:SpectraMax Plus. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. With optimized reagents, option to operate the. Italic and boldface type are used for emphasis. In . Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The advanced optical and. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The plate reader can measure multiple readouts, including UV. Type: Clear: Add to Cart. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Step 1: Turn on your microplate reader. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. Other technologies such as imaging. flat meniscus), but is approx 15 mL. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. San Jose, Calif. Temperature control and shaking come as standard. Use the provided 3. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. SpectraMax Plus: SpectraMax Plus. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. 4 μL DNA standards. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. We would like to show you a description here but the site won’t allow us. The SpectraMax® ABS Plus Microplate Reader can run . Endpoint, kinetic, spectrum. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Microplate Readers BioTek Epoch 2 Microplate Reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Sale. Sale. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. Absorbance (optical density, OD) was read at 570 nm and 605. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. . Time-. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. The price range of a reader starts at 2. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96-.